Course Content
🔬 Station 1 — Microscopic Identification of Acute Inflammation
Learning Outcomes Identify inflammatory cells on a provided microscopic slide. Identify the histopathological changes of acute appendicitis. Relate the demonstrated microscopic findings to acute inflammation.
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Microscopic Identification of Acute Inflammation
🔬 Station 2 — Microscopic Identification of Chronic and Granulomatous Inflammation
Learning Outcomes Identify the morphological changes of chronic inflammation in a provided specimen/slide. Identify the characteristic cells and their arrangement in a granuloma. Distinguish the demonstrated chronic inflammatory pattern from acute inflammation when asked.
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Microscopic Identification of Chronic and Granulomatous Inflammation
🔬 Station 3 — Identification of Granulation Tissue
Learning Outcomes Identify granulation tissue on a provided histopathology slide/image. Identify its characteristic microscopic features. Distinguish granulation tissue from granulomatous inflammation when asked.
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Identification of Granulation Tissue
🧪 Station 4 — Performance and Interpretation of Bacterial Biochemical Tests
Learning Outcomes Perform the bacterial biochemical test provided at the station using the appropriate technique. Observe and interpret the test result. Report the test as positive or negative based on the demonstrated reaction.
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Performance and Interpretation of Bacterial Biochemical Tests
🧫 Station 5 — Identification of Culture Media and Hemolysis on Blood Agar
Learning Outcomes Identify the provided microbiological culture medium. Recognize blood agar, mannitol salt agar, chocolate medium or Cary-Blair transport medium when presented. Identify the demonstrated pattern of hemolysis on blood agar.
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Identification of Culture Media and Hemolysis on Blood Agar
🚑 Station 6 — Demonstration of Gastric Lavage
Learning Outcomes Demonstrate the sequence of steps involved in gastric lavage using the provided model/manikin and equipment. Demonstrate safe handling and appropriate procedural sequence. State the essential precaution(s) relevant to the procedure when asked.
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Demonstration of Gastric Lavage
💊 Station 7 — Prescription Writing for Common Infectious Diseases Acute Tonsillitis / Malaria
Learning Outcomes Construct a complete prescription for the supplied infectious-disease scenario. Select appropriate medication with dose, route, frequency and duration. Demonstrate essential principles of safe and rational prescription writing.
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Prescription Writing for Common Infectious Diseases Acute Tonsillitis / Malaria
🦴🔍 Station 8 — Forensic Personal Identification: Bone or Hair/Fibre
Learning Outcomes Determine human sex from a provided bone or identify human hair microscopically, according to the station material. Identify the relevant distinguishing characteristics supporting the conclusion. Differentiate human hair from fibre when that specimen is provided.
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Forensic Personal Identification: Bone or Hair/Fibre
🦠 Station 9 — Laboratory Identification of Common Parasites
Learning Outcomes Identify the supplied parasite/specimen from a laboratory slide or specimen. Recognize the characteristic diagnostic form demonstrated. State the identifying feature supporting the diagnosis.
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Laboratory Identification of Common Parasites
🌍 Station 10 — Identification of Communicable Disease Models and Prevention
Learning Outcomes Identify the communicable disease represented by the provided model. Identify the important signs/features demonstrated by the model. State the major complication(s) and relevant preventive measures.
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Identification of Communicable Disease Models and Prevention
🧪🔬 AIM OSPE/OSCE Lab — Infection & Inflammation Module | 3rd Year MBBS
AIM OSCE / OSPE • MBBS

🔬 Station 1 — Microscopic Identification of Acute Inflammation

Rapid Revision • Histopathology Recognition • Viva

🎯 Task

CANDIDATE TASK

Examine the provided H&E-stained microscopic slide. Identify the predominant inflammatory cell, describe the important histopathological changes, and state the most likely pathological diagnosis and inflammatory process.

⚡ Model Station Answer

What to say in the exam

Identification: This slide shows acute appendicitis with acute suppurative inflammation.

Predominant cell: Neutrophils, recognized by their segmented multilobed nuclei.

Key features: Neutrophilic infiltration extending into the muscularis propria, with edema and vascular congestion; mucosal ulceration or necrosis may also be present.

Interpretation: These findings are characteristic of an acute inflammatory response.

🔎 Stepwise Procedure / Approach

  1. Start at low power. Identify the overall appendiceal wall and its mucosa, submucosa and muscularis propria.
  2. Assess the mucosa. Look for epithelial damage, ulceration or focal necrosis.
  3. Scan the wall. Look for edema, vascular dilatation and congestion.
  4. Move to higher power. Identify inflammatory cells by their nuclear morphology.
  5. Recognize neutrophils. Look for cells with dark segmented or multilobed nuclei and pale granular cytoplasm.
  6. Check the muscularis propria. Neutrophilic infiltration of this muscle layer is a key microscopic feature supporting acute appendicitis.
  7. Correlate and conclude. State acute appendicitis and relate the neutrophilic infiltrate, edema and vascular changes to acute inflammation.
Exam point: Neutrophils limited only to the mucosa are not sufficient by themselves for the classic histological diagnosis; neutrophilic infiltration of the muscularis propria is the important diagnostic feature.

🖼️ Visual Learning

Visual 1 — Acute Appendicitis: Recognizing Acute Inflammation on H&E

 

🎥 Practical Video

Acute Appendicitis: Microscopy
Source: Pathweb Teacher

A focused pathology demonstration showing the microscopic appearance of acute appendicitis and the features students should recognize on an H&E slide.

▶ Watch Practical Video

📝 Important Viva Questions

Q1. Which inflammatory cell predominates in acute inflammation?

Answer: Neutrophils are the predominant cells, particularly during the early phase of acute inflammation.

Q2. How do you identify a neutrophil microscopically?

Answer: It has a characteristically segmented multilobed nucleus, usually with three to five lobes, and pale cytoplasm containing fine granules.

Q3. What is the key histological feature of acute appendicitis?

Answer: Neutrophilic infiltration of the muscularis propria is the key diagnostic microscopic finding.

Q4. What vascular changes may be seen in acute inflammation?

Answer: Vasodilatation and vascular congestion occur, with increased vascular permeability producing tissue edema.

Q5. What additional microscopic changes may accompany acute appendicitis?

Answer: Mucosal ulceration or necrosis, submucosal edema, vascular congestion and fibrinopurulent serosal inflammation may be present.

Q6. What is meant by suppurative inflammation?

Answer: It is acute inflammation characterized by abundant neutrophils together with liquefactive tissue debris and formation of pus.

Q7. Which cells are more characteristic of chronic inflammation?

Answer: Chronic inflammation is predominantly associated with mononuclear cells, especially macrophages, lymphocytes and plasma cells.

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